SorbaRose™ – Beaded Cross-Linked
Agarose Solid Phase Resin

For immobilization of ligands under targeted purification using seven distinct coupling chemistries

SorbaRose is a family of activated agarose beads that enable you to direct purification protocols. An assortment of high performance, cross-linked agarose resins, and selected covalent binding technologies allow you to choose the most advantageous strategy for linking a ligand to the solid phase.

SorbaRose™ – Beaded Cross-Linked Agarose Solid Phase Resin

SorbaRose Solid Phase Agarose Resins:

  • Porous, stable, cross-linked beaded agarose provides a robust platform for both small and large scale applications
  • Tolerant of most Clean-In-Place (CIP) procedures based on the ligand
  • Choose from two different agarose concentrations (4 and 6%)
  • Available in seven distinct coupling chemistries
  • Applications include:
    • Antibody purification
    • High-resolution fractionation of biomolecules
    • Purification of human IgG
    • Immobilize enzymes, antibodies, and other proteins

SorbaRose is available in three different affinity and binding formats:

Affinity – For Purification of Biomolecules Using Affinity Chromatography

The available product categories are:

  • Purification of antibodies (matrices and columns with protein A, protein G, and protein L)
  • Purification of recombinant proteins with His-tag (plates and columns with IDA and NTA ligand for metal activation and finished products activated with nickel, cobalt, copper, and zinc)
  • Purification of recombinant proteins from GST (resin and glutathione columns)
  • Other products for affinity chromatography (beds and columns with biotin, heparin, and streptavidin)

Hydrophobic Interaction Chromatography (HIC) – For Purification of Biomolecules by Hydrophobic Interaction Chromatography Technique

  • In the HIC purification of biomolecules, even small differences in the hydrophobicity of the ligand can have a significant effect on the binding of the molecules.
  • In general, the hydrophobicity increases with the length of the ligand:
    Butyl < Pentyl < Hexyl < Heptyl < Octyl < Phenyl

Activated – Activated Beds for Covalent Binding of Ligands

  • A large selection of binding methods allows the best strategy to attach ligands to the substrate to be used, ensuring optimal conditions for the selected purification process.
  • Available with the following active groups:
    • Aldehyde
    • Amine
    • Carboxy
    • Epoxy
    • Hydrazide
    • NHS
    • Thiol