SorbaDex™ Dextran-Based Solid Phase Media (Compare to Cytiva Sephadex)

For the Separation of Biomolecules Based on Molecular Size

SorbaDex (compare to Cytiva Sephadex) is a beaded composite material comprised of ultrapure cross-linked dextran. It exhibits high selectivity, superb resolution, low non-specific adsorption, and robust chemical stability. Buffer and pH effects on resolution are minimal.

  • The main advantage of SorbaDex is the ability to rapidly remove small molecules and to simultaneously desalt the sample into pure water.
  • Superior performance, cost-effective replacement for Cytiva Sephadex™

SorbaDex is autoclavable at 121°C, pH 7 for 30 minutes and is stable in all commonly used buffers

SorbaDex™ Dextran-Based Solid Phase Media – Size Exclusion Chromatography
Grade Equivalent to Cytiva Sephadex G-25 Equivalent to Cytiva Sephadex G-50
Super Fine Dry Bead Size: 20 – 50 µm (> 80 %)

Water Regain: 2.15 – 2.25 mL/g

Swelling: 4 – 6 mL/g

MWCO (size exclusion): below 5000 Da Fractionation Range: 1 – 5 kDa (globular proteins) Catalog No.: 801001-01- 100gm 		Dry Bead Size: 20 – 50 µm (> 80 %)

Water Regain: 4.80 – 5.20 mL/g

Swelling: 9 – 11 mL/g

MWCO (size exclusion): below 25000 Da
Fractionation Range: 1 – 30 kDa (globular proteins)
Catalog No.: 801004-01-100gm
Fine Dry Bead Size: 20 – 80 µm (> 80 %)

Water Regain: 2.15 – 2.25 mL/g

Swelling: 4 – 6 mL/g

MWCO (size exclusion): below 5000 Da Fractionation Range: 1 – 5 kDa (globular proteins) Catalog No.: 801002-01 -100gm 		Dry Bead Size: 20 – 80 µm (> 80 %)

Water Regain: 4.80 – 5.20 mL/g

Swelling: 9 – 11 mL/g

MWCO (size exclusion): below 25000 Da
Fractionation Range: 1 – 30 kDa (globular proteins)
Catalog No.: 801005-01 -100gm
Medium Dry Bead Size: 50 – 150 µm (> 80%)

Water Regain: 2.15 – 2.25 mL/g

Swelling: 4 – 6 mL/g

MWCO (size exclusion): below 5000 Da Fractionation Range: 1 – 5 kDa (globular proteins) Catalog No.: 801003-01 -100gm 		Dry Bead Size: 50 – 150 µm (> 80%)

Water Regain: 4.80 – 5.20 mL/g

Swelling: 9 – 11 mL/g

MWCO (size exclusion): below 25000 Da
Fractionation Range: 1 – 30 kDa (globular proteins)
Catalog No.: 801006-01 -100gm
Catalog No.DescriptionPrice (USD)
801001-01SorbaDex Gel Filtration Matrix, S-25 Superfine, 100g Inquire
801001-05SorbaDex Gel Filtration Matrix, S-25 Superfine, 500g Inquire
801001-1SorbaDex Gel Filtration Matrix, S-25 Superfine, 1kg Inquire
801001-5SorbaDex Gel Filtration Matrix, S-25 Superfine, 5kg Inquire
801002-01SorbaDex Gel Filtration Matrix, S-25 Fine, 100g Inquire
801002-05SorbaDex Gel Filtration Matrix, S-25 Fine, 500g Inquire
801002-1SorbaDex Gel Filtration Matrix, S-25 Fine, 1kg Inquire
801002-5SorbaDex Gel Filtration Matrix, S-25 Fine, 5kg Inquire
801003-01SorbaDex Gel Filtration Matrix, S-25 Medium, 100g Inquire
801003-05SorbaDex Gel Filtration Matrix, S-25 Medium, 500g Inquire
801003-1SorbaDex Gel Filtration Matrix, S-25 Medium, 1kg Inquire
801003-5SorbaDex Gel Filtration Matrix, S-25 Medium, 5kg Inquire
801004-01SorbaDex Gel Filtration Matrix, S-50 Superfine, 100g Inquire
801004-05SorbaDex Gel Filtration Matrix, S-50 Superfine, 500g Inquire
801004-1SorbaDex Gel Filtration Matrix, S-50 Superfine, 1kg Inquire
801004-5SorbaDex Gel Filtration Matrix, S-50 Superfine, 5kg Inquire
801005-01SorbaDex Gel Filtration Matrix, S-50 Fine, 100g Inquire
801005-05SorbaDex Gel Filtration Matrix, S-50 Fine, 500g Inquire
801005-1SorbaDex Gel Filtration Matrix, S-50 Fine, 1kg Inquire
801005-5SorbaDex Gel Filtration Matrix, S-50 Fine, 5kg Inquire
801006-01SorbaDex Gel Filtration Matrix, S-50 Medium, 100g Inquire
801006-05SorbaDex Gel Filtration Matrix, S-50 Medium, 500g Inquire
801006-1SorbaDex Gel Filtration Matrix, S-50 Medium, 1kg Inquire
801006-5SorbaDex Gel Filtration Matrix, S-50 Medium, 5kg Inquire
Catalog No. Fractionation range (globular proteins) – Mr pH stability Bed volume ml/g dry SorbaDex™ Bead size (dry) Product
801001
1-5 kDa
2 to 13
4 – 6
20-50 µm
SorbaDex™ – 25 superfine
801002
1-5 kDa
2 to 13
4 – 6
20-80 µm
SorbaDex™ – 25 fine
801003
1-5 kDa
2 to 13
4 – 6
50-150 µm
SorbaDex™ – 25 medium
801004
1-30 kDa
2 to 13
9 – 11
20-50 µm
SorbaDex™ – 50 superfine
801005
1-30 kDa
2 to 13
9 – 11
20-80 µm
SorbaDex™ – 50 fine
801006
1-30 kDa
2 to 13
9 – 11
50-150 µm
SorbaDex™ – 50 medium
801007
Hydrated in phosphate buffered saline; pH 7.4, with 0.2% sodium azide
SorbaDex™ – 50 fine
801008
SorbaDex™ – 25 medium
High Performance Results

  • Sample: Mouse IgG in PBS
  • Conc.: 12.5 mg/ml
  • Volume: 4500 ml
  • Total IgG: 56.25 grams
  • Buffer: 100 mM Glycine-HCl, pH 2.0 neutralised with 1 M Tris-HCl pH 8.0
  • System: ÄktaPilot (GE Healthcare)
  • Column: Sorbadex-25 M in BPG 300/500
  • Diameter: 30 cm
  • Gel Volume: 25 Liters
  • Pressure: 1.0 bar (0.1 mPa or 13 psi)
  • Flow rate during sample loading: 400 ml
  • Flow rate during equilibation and elution: 500 ml
  • Total run time: 63 min
Protein Desalting from 0.8M NaCl
IgG (280nm): dark blue line
NaCl (µS): gold line
1 mg IgG anti-Rabbit in 1 mL 0.8 M sodium chloride
Water elution
Removal of FITC from IgG
IgG (280nm): dark blue line
FITC (550nm): gold line
1 mg IgG anti-Rabbit and 0.1 µmol FITC in 1mL DMSO/NaHCO3
PBS Elution
Oligo Desalting from 0.8M NaCl
Oligo (260nm): dark blue line
NaCl (µS): gold line
1 mg oligonucleotide (18-mer) in 1 mL 0.8 M NaCl
Removal of Rhodamine after labeling
Oligo (260nm): dark blue line
TAMRA (550nm): gold line
1 mg oligonucleotide (18-mer) and 0.5 µmol TAMRA in 1 mL DMSO/NaHCO3
Removal of Ammonia (33%)
Dextran Blue (260nm): dark blue line
NH3 (pH): gold line
0.5 mg Dextran Blue (Mr 2,000,000) in 1 mL ammonia (33%)
Water elution
Removal of Ammonia (33%) from Oligo
Oligo (260nm): dark blue line
NH3 (pH): gold line
1 mg oligonucleotide (18-mer) in 1 mL ammonia (33%) after cleavage