CHROMABOND® XTR – SPE Cartridges

Kieselguhr phase for liquid-liquid extraction

Base material coarse-grained kieselguhr (also known as diatomaceous earth, hydromatrix, celite) large pore size, high pore volume, constantly high batch-to-batch quality pH working range 1-13

Application:

  • Liquid-liquid extraction of highly viscous aqueous solutions such as physiological fluids (blood, plasma, and serum) in clinical chemistry, dyes in textiles, environmental and food analysis without the use of a separation funnel
  • High water loadability without a breakthrough of water during elution with
    organic solvents
  • Also suited for removing small amounts of water from solvents which are not miscible with water

Advantages:

  • Fast, reproducible, and economical simultaneous preparation of several samples
  • No problems with phase separation
  • No formation of emulsions
  • High recovery rates
  • Saving of time and solvents
  • Organic solutions need not be dried after separation
SPE CHROMABOND PP columns XTR

CHROMABOND® XTR

Solvents applicable for elution
  • Diethyl ether
  • tert butyl methyl ether
  • Ethyl acetate
  • n-hexane
  • Cyclohexane
  • Toluene
  • Dichloromethane (methylene chloride)
  • Trichloromethane (chloroform)
  • Trichloromethane – methanol (90:10, v/v)
  • Trichloromethane – methanol (85:15, v/v)
  • Diethyl ether – ethanol (90:10, v/v)
  • Diethyl ether – ethanol (80:20, v/v)
  • Dichloromethane – 2-propanol (90:10, v/v)
  • Dichloromethane – 2-propanol (85:15, v/v)
Eluents with too high alcohol contents cause an increase in volume of the aqueous phase on the CHROMABOND® XTR. Here the column could be overloaded and the aqueous phase displaced from the column. In this case, a greater capacity column should be used.
Depending on the concentration of the analytes, eluates can be analyzed immediately, or the organic solvent is evaporated. The pH value of the aqueous solution can be altered on the column, which enables elution of different compounds of a sample under optimized conditions. Under certain circumstances, acidic, neutral, and basic compounds can be fractionated in this way.