NUCLEOGEN^® DEAE

The Nucleogen family offers remarkable performance for biopolymers. It is a series of silica-based DEAE anion exchangers available with pore sizes of 60, 500 and 4000 Å, respectively. The choice of pore sizes and the chemistry of the surface coating is best suited for separation of oligonucleotides, high molecular weight RNA and plasmid DNA.

Base material silica, particle size 7 µm
(SiO2)n – Spacer – N CH3
DEAE anion exchanger

Nucleogen 60-7 DEAE: pore size 60 Å

• For separation of oligonucleotides up to chain lengths of 40 bases with recoveries > 95 % capacity 200 A260/ml (~ 300 A260 for a 125 x 4 mm ID column, 1875 A260 for a 125 x 10 mm ID column)
• Preparative separations possible when using higher flow rates and longer gradient times

Nucleogen 500-7 DEAE: pore size 500 Å

• For the separation of tRNA, 5S RNA, viroids and messenger RNA in the intermediate molecular weight range (25,000-1,000,000 daltons) with recoveries > 95%
• Capacity 730 A260 for a 125 x 6 mm ID column, 1940 A260 for a 125 x 10 mm ID column

Nucleogen 4000-7 DEAE: pore size 4000 Å

• For the separation of plasmids, DNA restriction fragments, ribosomal RNA, messenger RNA and viral RNA, i. e. very high molecular weight nucleic acids (e. g. 1-50 megadaltons)
• Capacity 120 A260 for a 125 x 6 mm ID column, 350 A260 for a 125 x 10 mm ID column
• Eluent in column methanol