NUCLEODUR^® – Cyano-Nitrile

In reversed-phase HPLC it’s fairly common to start with C18 or C8 columns if new methods must be developed.

Superior polarity and selectivity properties often required for more sophisticated separations, aren’t always sufficiently provided by classical RP phases, characterized by a hydrophobic layer of monomeric or polymeric bonded alkylsilanes.

Improve the resolution of compounds separated on nonpolar stationary phases, change bonded-phase functionality.

The fully endcapped and highly reproducible NUCLEODUR 100-5 CN-RP phase has cyanopropyl groups on the
surface able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications.

The polarity of the NUCLEODUR 100-5 CN-RP phase can be classified as intermediate based on multiple retention mechanisms such as dipole-dipole, p-p, and also hydrophobic interactions [C. S. Young and R. J. Weigand, LCGC 20 (2002) 464-473].

This phase shows a distinct selectivity for polar organic compounds as well as for molecules containing p electron systems (e. g. analytes with double bonds, tricyclic antidepressants) [V. R. Meyer, Practical High-Performance Liquid Chromatography (John Wiley & Sons, New York, 3rd. ed., 1999)].

Short-chain bonded phases are sometimes suspected of revealing shortcomings instability towards hydrolysis at low pH [J. J. Kirkland, LCGC 14 (1996) 486-500].

NUCLEODUR CN columns for normal phase applications are shipped in n-heptane. The high coverage combined with endcapping makes NUCLEODUR 100-5 CN-RP suitable for the separation of ionizable compounds such as basic drugs.