NUCLEODUR^® – C18 EC & C8 EC

The utmost purity of the base silica and the exceptional silane bonding chemistry minimizes the risk of dissolution or hydrolysis at pH extremes. The chromatograms show the retention behavior at pH values of 1.5 and 10.0 for NUCLEODUR^® 100-5 C18 ec

Loadability, probably the most important feature for preparative LC applications, is determined by pore size, pore volume and surface area of the packing. However, it can also be influenced by the molecular weight of the analytes.

NUCLEODUR C8 Gravity and NUCLEODUR^® C8ec columns expand the reversed-phase toolbox effectively. Based on the same totally spherical and highly pure silica the C8 phases exhibit the same excellent chemical and mechanical stability features as the C18 counterparts.

NUCLEODUR C8 Gravity can also be run at pH extremes (pH 1-11) by choosing appropriate elution parameters. Due to the shorter chain and less hydrophobic properties of the stationary phase, the retention of non-polar compounds is decreased, and in consequence, a reduction in time of analysis can be achieved.

A stronger polar selectivity, particularly with the separation of ionizable analytes is frequently observed (as distinct from the C18 phases). NUCLEODUR C8 ec and NUCLEODUR^® C8 Gravity are most suitable for the development of new methods but also for robust routine analysis.

C18 or C8-The best of both worlds

Regarding the differences between C8 and C18 phases and the preferred range of applications, there are no general guidelines that could make the choice easier but it will always be beneficial to add both phases to the existing pool of reversed-phase columns in the laboratory.