SorbaSep™ FPLC Desalting Columns
(Cytiva Compatible)
For Desalting Using FPLC Systems – Including Cytiva AKTATM, Bio-Rad NGCTM, and Knauer AZURATM
Cytiva compatible SorbaSep desalting columns are used for biomolecule purifications from smaller molecule contamination, buffer exchange, and desalting.
They are fully compatible with all FPLC and liquid chromatography systems and offer a simple, rapid and reproducible process for purification of your sample.
SorbSep FPLC Column Features:
- Preloaded with SorbaDex-25 Superfine, a beaded composite size-exclusion matrix. It exhibits high flow rates, excellent resolution, and chemical stability
- Fractionation range for globular proteins from 1 and 5 kD, aqueous compatible, pH range of 2-13
- 5mL column bed volume for use with samples from 0.1 to 1.5mL for full removal of contaminants
- Columns can be connected in series to permit large sample volume processing
- Cost-effective, quality performance alternative to HiTrap™, Bio-Scale™ P6, Zeba™ Desalting, and more
| Catalog No. | Description | Price (USD) |
|---|---|---|
| 803028-5 | SorbaSep FPLC Desalting Columns, with Sorbadex S-25 Superfine for buffer exchange and/or desalting using a syringe, a pump, or a chromatography system. 5.0 ml, 5 columns |  Inquire |
| 803028-100 | SorbaSep FPLC Desalting Columns, with Sorbadex S-25 Superfine for buffer exchange and/or desalting using a syringe, a pump, or a chromatography system. 5.0 ml, 100 columns | Inquire |
| Specifications | |
|---|---|
| Column Bed Volume | 5 ml |
| Size of Eluted Proteins | > 5 kD |
| System Compatibility | - Automated liquid chromatography systems (MPLC, FPLC™, ÄKTA™,etc.) - Peristaltic Pump - Syringe |
| Column Dimensions | 1.6 cm inner diameter x 2.5 cm height |
| Column Body Material | Polypropylene |
| Column Ports | Inlet 10–32 (1/16”) female Outlet 10–32 (1/16”) male |
| Support | Sorbadex™-25 Superfine |
| Bead size | 40–110 μm (hydrated) |
| Maximum Back Pressure | 3 bar (0.3 MPa) |
| Recommended Flow Rate | 1 to 5 ml/min |
| Maximum Recommended Flow Rate | 10 ml/min |
| Storage Temperature | ambient |
| Storage Solution | 20 % (v/v) ethanol |
SorbaSep FPLC Desalting Column Datasheet (PDF)
SorbaSep™ FPLC Desalting Columns
Optimize your purification and chromatography with Sorbtech’s precision engineered SorbaSep™ FPLC columns. Each allows for easy packing, reproducibility, reliability, and scalability to increase productivity in your lab. Use SorbaSep™ FPLC columns to desalt, remove small molecules, and buffer exchange in standalone or automated HPLC systems—and for separating larger biomolecules from unwanted smaller molecules. Simple, rapid, and reproducible separation using a syringe, pump, or liquid chromatography system.
The fractionation range for globular proteins is between 1 and 5 kD. The size exclusion cut-off is approximately 5 kD, which ensures efficient separation of proteins/peptides/biomolecules larger than 5 kD from lower molecular weight molecules of less than 1 kD. SorbaSep™ desalting FPLC columns contain SorbaDex™-25 Superfine, a beaded composite size exclusion matrix. It exhibits high flow rates, excellent resolution, and chemical stability. Buffer and pH effects on resolution are minimal.
