Clarion™ Series – Hydrated Gel Filtration Columns for Purification, Desalting, and Buffer Exchange

For rapid gravity purification, desalting, and buffer exchange

Designed to rapidly and efficiently remove small molecules (salts, dyes, ammonia, haptens, biotin) from large molecules (antibodies, enzymes, and other proteins). High performance, lower cost replacement of Cytiva MidiTrap™ columns.

  • Ultrapure gel and specially treated sinter frits ensure:
    • Outstanding resolution
    • Minimized cross-contamination
    • High selectivity
  • The gel matrix is SorbaDex™-25, a beaded composite material comprised of ultrapure cross-linked dextran optimized for gravity flow
Clarion™ Series – Hydrated Gel Filtration Columns for Purification, Desalting, and Buffer Exchange

Easy four-step protocol

Clarion Series Hydrated Gel Filtration Columns

Clarion-N Gel Filtration Columns

For rapid removal of small molecules from nucleic acids

  • Use N columns for longer than 10 base pairs/nucleotides
  • Use NF columns for longer than 20 base pairs/nucleotides
Clarion Protein Desalting Columns N

Clarion-N performance removal of fluorescent dye
2.5ml sample volume-elution with pure water

Clarion-P Gel Filtration Columns

Removal of small molecules from antibodies, enzymes and other proteins

  • Use P columns for protein >5kD and nanoparticles > 2nm
  • Use PF columns for proteins >25kD and nanoparticles > 4nm
Clarion Protein Desalting Columns P

Clarion-P performance-desalting of protein solution
1.0mL sample vol., elution with pure water

Clarion N-Series

Catalog No.DescriptionPrice (USD)
803010-2Clarion N2 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes 150-300 uL, 2 columns Inquire
803010-50Clarion N2 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes 150-300 uL, 50 columns Inquire
803011-2Clarion N5 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes 100-500 uL, 2 columns Inquire
803011-50Clarion N5 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes 100-500 uL, 50 columns Inquire
803012-2Clarion N10 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes 0.5-1.0 mL, 2 columns Inquire
803012-50Clarion N10 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes 0.5-1.0 mL, 50 columns Inquire
803013-2Clarion NF10 Gel Filtration Columns, N10 with Sorbadex S-50 Medium, 50ml, 2 columns Inquire
803013-50Clarion NF10 Gel Filtration Columns, N10 with Sorbadex S-50 Medium, 50ml, 50 columns Inquire
803014-2Clarion N25 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes up to 2.5 mL, 2 columns Inquire
803014-25Clarion N25 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes up to 2.5 mL, 25 columns Inquire
803015-1Clarion N50 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes up to 5.0 mL, 1 column Inquire
803015-10Clarion N50 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes up to 5.0 mL, 10 columns Inquire
803017-1Clarion N100 Columns, With Sorbadex S-25 Medium For Desalting, Buffer Exchange, and/or Remove Free Label, 10ml, 1 column Inquire
803017-10Clarion N100 Columns, With Sorbadex S-25 Medium For Desalting, Buffer Exchange, and/or Remove Free Label, 10ml, 10 columns Inquire
803018-1Clarion N500 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes up to 50.0 mL, 1 column Inquire

Clarion P-Series

Catalog No.DescriptionPrice (USD)
803019-2Clarion P2 Columns, hydrated gel filtration column for protein purification; for sample volumes 150-300 uL, 2 columns Inquire
803019-50Clarion P2 Columns, hydrated gel filtration column for protein purification; for sample volumes 150-300 uL, 50 columns Inquire
803020-2Clarion P5 Columns, hydrated gel filtration column for protein purification; for sample volumes 100-500 uL, 2 columns Inquire
803020-50Clarion P5 Columns, hydrated gel filtration column for protein purification; for sample volumes 100-500 uL, 50 columns Inquire
803021-2Clarion P10 Columns, hydrated gel filtration column for protein purification; for sample volumes 0.5-1.0 mL, 2 columns Inquire
803022-2Clarion P25 Columns, hydrated gel filtration column for protein purification; for sample volumes up to 2.5 mL, 2 columns Inquire
803022-25Clarion P25 Columns, hydrated gel filtration column for protein purification; for sample volumes up to 2.5 mL, 25 columns Inquire
803023-1Clarion P50 Columns, hydrated gel filtration column for protein purification; for sample volumes up to 5.0 mL, 1 column Inquire
803023-10Clarion P50 Columns, hydrated gel filtration column for protein purification; for sample volumes up to 5.0 mL, 10 columns Inquire
803025-1Clarion P100 Columns, hydrated gel filtration column for protein purification; for sample volumes up to 10.0 mL, 1 column Inquire
803025-10Clarion P100 Columns, hydrated gel filtration column for protein purification; for sample volumes up to 10.0 mL, 10 columns Inquire

Clarion P-Series
Hydrated gel filtration columns for protein purification and desalting.

  • Clarion P2 processes sample volumes of 150 to 300 µL
  • Clarion P5 processes sample volumes of 0.5 mL
  • Clarion P10 processes sample volumes of 0.5 mL to 1.0 mL
  • Clarion P25 processes sample volumes of 2.5 mL
  • Clarion P50 processes sample volumes of 5 mL
  • Clarion P100 processes sample volumes of 10 mL

Clarion Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins.

The gel matrix of Clarion is Sorbadex-25, a beaded composite material composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Sorbadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Sorbadex-25 is 5 kD for proteins.

  • Clarion P2 – Proteins larger than 5 kD in a sample volume of up to 200 µL can be purified with an elution volume of 200 to 350 µL.
  • Clarion P5 – Proteins larger than 5 kD in a sample volume of up to 0.5 mL can be purified with an elution volume of up to 1.0 mL.
  • Clarion P10 – Proteins larger than 5 kD in a sample volume of up to 1 mL can be purified with an elution volume of 1.2 to 1.5 mL.
  • Clarion P25 – Proteins larger than 5 kD in a sample volume of up to 2.5 mL can be purified with an elution volume of 2.7 to 3.5 mL.
  • Clarion P50 – Proteins larger than 5 kD in a sample volume of 5 mL can be purified with an elution volume of 6 to 8 mL.
  • Clarion P100 – Proteins larger than 5 kD in a sample volume of 10 mL can be purified with an elution volume of 12 to 15 mL.

Hydrated gel filtration columns for nucleic acid purification and desalting.

  • Clarion N2 processes sample volumes of 150 to 300 µL
  • Clarion N5 processes sample volumes of 0.5 mL
  • Clarion N10 processes sample volumes of 0.5 mL to 1.0 mL
  • Clarion N25 processes sample volumes of 2.5 mL
  • Clarion N50 processes sample volumes of 5 mL
  • Clarion N100 processes sample volumes of 10 mL
  • Clarion N500 processes sample volumes of 50 mL

The gel matrix of Clarion is Sorbadex-25, a beaded composite material composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability.

Molecules purified with Sorbadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal.

N2 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from nucleic acids.

The gel matrix of Clarion is Sorbadex-25, a beaded composite material composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Sorbadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Sorbadex-25 is 10 base pairs for oligonucleotides.

  • Clarion N2 – Nucleic acids larger than 10 bp in a sample volume of up to 200 µL can be purified with an elution volume of 200 to 350 µL.
  • Clarion N5 – Nucleic acids larger than 10 bp in a sample volume of up to 0.5 mL can be purified with an elution volume of up to 1.0 mL.
  • Clarion N10 – Nucleic acids larger than 10 bp in a sample volume of up to 1 mL can be purified with an elution volume of 1.2 to 1.5 mL.
  • Clarion N25 – Nucleic acids larger than 10 bp in a sample volume of up to 2.5 mL can be purified with an elution volume of 2.7 to 4.0 mL.
  • Clarion N50 – Nucleic acids larger than 10 bp in a sample volume of 5 mL can be purified with an elution volume of 6 to 8 mL.
  • Clarion N100 – Nucleic acids larger than 10 bp in a sample volume of 10 mL can be purified with an elution volume of 12 to 15 mL.
  • Clarion N100 – Nucleic acids larger than 10 bp in a sample volume of 50 mL can be purified with an elution volume of 65 to 70 mL.