Sorbadex™ Media, Gel Filtration Matrix



For Process Purification of Proteins and Nucleic Acids

Sorbadex Media, Gel Filtration Matrix, (Like GE's Sephadex)

SorbaDex™ is a beaded composite material composed partially of cross-linked dextran. It exhibits high selectivity, high resolution and chemical stability.

SorbaDex™ is a size exclusion matrix. Molecules purified with SorbaDex™ are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal.

The size exclusion cut-off for SorbaDex™-25 is set at 5 kD for proteins and 10 bp for nucleic acids. For SorbaDex™-50, the cut-offs are 25 kD and 20 bp. Purified biomolecules are not significantly diluted when processed using SorbaDex™.

SorbaDex™ bulk powders are available in 100g, 500g, 1kg, 5kg, 10kg, and 100kg package sizes.






Sorbadex Gel Filtration Matrix

Catalog #/DescriptionAdd to Cart/List Price
801001-01 - SorbaDex Gel Filtration Matrix, S-25 Superfine, 100gAdd to Cart  331.25
801001-05 - SorbaDex Gel Filtration Matrix, S-25 Superfine, 500gAdd to Cart  1437.50
801001-1 - SorbaDex Gel Filtration Matrix, S-25 Superfine, 1kgCALL FOR PRICE
801001-5 - SorbaDex Gel Filtration Matrix, S-25 Superfine, 5kgCALL FOR PRICE
801002-01 - SorbaDex Gel Filtration Matrix, S-25 Fine, 100gAdd to Cart  262.50
801002-05 - SorbaDex Gel Filtration Matrix, S-25 Fine, 500gAdd to Cart  1118.75
801002-1 - SorbaDex Gel Filtration Matrix, S-25 Fine, 1kgCALL FOR PRICE
801002-5 - SorbaDex Gel Filtration Matrix, S-25 Fine, 5kgCALL FOR PRICE
801003-01 - SorbaDex Gel Filtration Matrix, S-25 Medium, 100gAdd to Cart  256.25
801003-05 - SorbaDex Gel Filtration Matrix, S-25 Medium, 500gAdd to Cart  1087.50
801003-1 - SorbaDex Gel Filtration Matrix, S-25 Medium, 1kgCALL FOR PRICE
801003-5 - SorbaDex Gel Filtration Matrix, S-25 Medium, 5kgCALL FOR PRICE
801004-01 - SorbaDex Gel Filtration Matrix, S-50 Superfine, 100gAdd to Cart  375.00
801004-05 - SorbaDex Gel Filtration Matrix, S-50 Superfine, 500gAdd to Cart  1593.75
801004-1 - SorbaDex Gel Filtration Matrix, S-50 Superfine, 1kgCALL FOR PRICE
801004-5 - SorbaDex Gel Filtration Matrix, S-50 Superfine, 5kgCALL FOR PRICE
801005-01 - SorbaDex Gel Filtration Matrix, S-50 Fine, 100gAdd to Cart  281.25
801005-05 - SorbaDex Gel Filtration Matrix, S-50 Fine, 500gAdd to Cart  1187.50
801005-1 - SorbaDex Gel Filtration Matrix, S-50 Fine, 1kgCALL FOR PRICE
801005-5 - SorbaDex Gel Filtration Matrix, S-50 Fine, 5kgCALL FOR PRICE
801006-01 - SorbaDex Gel Filtration Matrix, S-50 Medium, 100gAdd to Cart  312.50
801006-05 - SorbaDex Gel Filtration Matrix, S-50 Medium, 500gAdd to Cart  1312.50
801006-1 - SorbaDex Gel Filtration Matrix, S-50 Medium, 1kgCALL FOR PRICE
801006-5 - SorbaDex Gel Filtration Matrix, S-50 Medium, 5kgCALL FOR PRICE


Sorbadex Lipophilic Hydrophilic Gel Filtration Matrix

Catalog #/DescriptionAdd to Cart/List Price
801009-0025 - SorbaDex Lipophilic Hydrophilic Gel Filtration Matrix, 20-LH, 25gAdd to Cart  141.88
801009-01 - SorbaDex Lipophilic Hydrophilic Gel Filtration Matrix, 20-LH, 100gAdd to Cart  429.19
801009-05 - SorbaDex Lipophilic Hydrophilic Gel Filtration Matrix, 20-LH, 500gAdd to Cart  1965.64
801009-1 - SorbaDex Lipophilic Hydrophilic Gel Filtration Matrix, 20-LH, 1kgCALL FOR PRICE
Cat. No.Fractionation range
(globular proteins) - Mr
pH stabilityBed volume ml/g dry Sorbadex™Bead size (dry)Product
8010011-5 kDa2 to 134 - 620-50 µmSorbadex™-25 superfine
8010021-5 kDa2 to 134 - 620-80 µmSorbadex™-25 fine
8010031-5 kDa2 to 134 - 650-150 µmSorbadex™-25 medium
8010041-30 kDa2 to 139 - 1120-50 µmSorbadex™-50 superfine
8010051-30 kDa2 to 139-1120-80 µmSorbadex™-50 fine
8010061-30 kDa2 to 139-1150-150 µmSorbadex™-50 medium
801007Hydrated in phosphate buffered saline; pH 7.4,with 0.2% sodium azideSorbadex™-50 fine
801008Sorbadex™-25 medium

High Performance Results

  • Sample: Mouse IgG in PBS
  • Conc.: 12.5 mg/ml
  • Volume: 4500 ml
  • Total IgG: 56.25 grams
  • Buffer: 100 mM Glycine-HCl, pH 2.0 neutralised with 1 M Tris-HCl pH 8.0
  • System: ÄktaPilot (GE Healthcare)
  • Column: Sorbadex-25 M in BPG 300/500
  • Diameter: 30 cm
  • Gel Volume: 25 Liters
  • Pressure: 1.0 bar (0.1 mPa or 13 psi)
  • Flow rate during sample loading: 400 ml
  • Flow rate during equilibation and elution: 500 ml
  • Total run time: 63 min

Protein Desalting from 0.8M NaCl
IgG (280nm): dark blue line
NaCl (µS): gold line
1 mg IgG anti-Rabbit in 1 mL 0.8 M sodium chloride
Water elution

Removal of FITC from IgG
IgG (280nm): dark blue line
FITC (550nm): gold line
1 mg IgG anti-Rabbit and 0.1 µmol FITC in 1mL DMSO/NaHCO3
PBS Elution

Oligo Desalting from 0.8M NaCl
Oligo (260nm): dark blue line
NaCl (µS): gold line
1 mg oligonucleotide (18-mer) in 1 mL 0.8 M NaCl

Removal of Rhodamine after labeling
Oligo (260nm): dark blue line
TAMRA (550nm): gold line
1 mg oligonucleotide (18-mer) and 0.5 µmol TAMRA in 1 mL DMSO/NaHCO3

Removal of Ammonia (33%)
Dextran Blue (260nm): dark blue line
NH3 (pH): gold line
0.5 mg Dextran Blue (Mr 2,000,000) in 1 mL ammonia (33%)
Water elution

Removal of Ammonia (33%) from Oligo
Oligo (260nm): dark blue line
NH3 (pH): gold line
1 mg oligonucleotide (18-mer) in 1 mL ammonia (33%) after cleavage