Clarion™ P-Series Gel Filtration Columns (Gravity Flow)

  • Clarion™ P-Series Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins.
  • Clarion™ P-Series Columns are specifically designed for rapid and efficient removal of small molecules from antibodies, enzymes and other proteins.
  • Ultrapure gel and specially treated sinter frits ensure outstanding resolution and high selectivity.
  • The gel matrix is Sorbadex™-25, a beaded composite material comprised of ultrapure cross-linked dextran. It exhibits high selectivity, high resolution and chemical stability.
  • Molecules purified with Sorbadex™-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules.
  • Buffer and pH effects on resolution are minimal. The molecular weight cut-off (MWCO) for Sorbadex™-25 is 5 kD for proteins. Proteins larger than 5 kD are typically purified with a 1.5-fold elution volume.

    Clarion™ MINI Spin Columns

  • Clarion™ MINI Spin Columns are used for quick and efficient desalting, buffer exchange, dye terminator removal and removal of labels from labeling reactions. The columns are sterile packed, pre-swollen with purified water and ready-to-use.
  • Clarion™ MINI Spin Columns are designed for purification and desalting of oligonucleotides longer than 20 base pairs and from proteins greater than 20 kD. Convenient centrifugation protocol results in minimal dilution.
  • Clarion™ MINI Spin Columns process samples having a volume between 10 and 100 µL. Optimal purification and recovery is obtained with a sample volume of 50 µL. The volume of the gel bed is 0.8 mL.




Desalt

Catalog #/DescriptionAdd to Cart/List Price
803001-4 - Clarion MINI Spin Columns, Desalt S-25, hydrated with pure, deionized water for quick and efficient desalting, buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD, 4 columnsAdd to Cart  19.38
803001-25 - Clarion MINI Spin Columns, Desalt S-25, hydrated with pure, deionized water for quick and efficient desalting, buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD, 25 columnsAdd to Cart  89.13
803006-25 - Clarion MINI Spin Columns, Desalt S-50, hydrated with pure, deionized water for quick and efficient desalting, buffer exchange and/or removal of dyes and small molecules from proteins greater than 25 kD, 25 columnsAdd to Cart  89.13
803006-100 - Clarion MINI Spin Columns, Desalt S-50, hydrated with pure, deionized water for quick and efficient desalting, buffer exchange and/or removal of dyes and small molecules from proteins greater than 25 kD, 100 columnsAdd to Cart  328.33


Desalt AZ

Catalog #/DescriptionAdd to Cart/List Price
803005-4 - Clarion MINI Spin Columns, Desalt S-25AZ, hydrated with pure deionized water and stabilized with 0.02% sodium azide for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD, 4 columnsAdd to Cart  19.38
803005-25 - Clarion MINI Spin Columns, Desalt S-25AZ, hydrated with pure deionized water and stabilized with 0.02% sodium azide for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD, 25 columnsAdd to Cart  89.13
803005-100 - Clarion MINI Spin Columns, Desalt S-25AZ, hydrated with pure deionized water and stabilized with 0.02% sodium azide for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD, 100 columnsAdd to Cart  328.33


Tris

Catalog #/DescriptionAdd to Cart/List Price
803003-4 - Clarion MINI Spin Columns, TRIS S-25, hydrated with 1 mM TRIS pH 6 for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD, 4 columnsAdd to Cart  19.38
803003-25 - Clarion MINI Spin Columns, TRIS S-25, hydrated with 1 mM TRIS pH 6 for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD, 25 columnsAdd to Cart  89.13
803003-100 - Clarion MINI Spin Columns, TRIS S-25, hydrated with 1 mM TRIS pH 6 for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD, 100 columnsAdd to Cart  328.33
803008-4 - Clarion MINI Spin Columns, TRIS S-50, hydrated with 1 mM TRIS pH 6 for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 25 kD, 4 columnsAdd to Cart  19.38
803008-25 - Clarion MINI Spin Columns, TRIS S-50, hydrated with 1 mM TRIS pH 6 for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 25 kD, 25 columnsAdd to Cart  89.13
803008-100 - Clarion MINI Spin Columns, TRIS S-50, hydrated with 1 mM TRIS pH 6 for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 25 kD, 100 columnsAdd to Cart  328.33


PBS

Catalog #/DescriptionAdd to Cart/List Price
803004-4 - Clarion MINI Spin Columns, PBS S-25, hydrated with Phosphate Buffered Saline pH 7 for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD, 4 columnsAdd to Cart  19.38
803004-25 - Clarion MINI Spin Columns, PBS S-25, hydrated with Phosphate Buffered Saline pH 7 for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD, 25 columnsAdd to Cart  89.13
803004-100 - Clarion MINI Spin Columns, PBS S-25, hydrated with Phosphate Buffered Saline pH 7 for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD, 100 columnsAdd to Cart  328.33
803009-4 - Clarion MINI Spin Columns, PBS S-50, hydrated with Phosphate Buffered Saline pH 7 for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 25 kD, 4 columnsAdd to Cart  19.38
803009-25 - Clarion MINI Spin Columns, PBS S-50, hydrated with Phosphate Buffered Saline pH 7 for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 25 kD, 25 columnsAdd to Cart  89.13
803009-100 - Clarion MINI Spin Columns, PBS S-50, hydrated with Phosphate Buffered Saline pH 7 for rapid buffer exchange and/or removal of dyes and small molecules from proteins greater than 25 kD, 100 columnsAdd to Cart  328.33


Clarion P-series

Catalog #/DescriptionAdd to Cart/List Price
803019-2 - Clarion P2 Columns, hydrated gel filtration column for protein purification; for sample volumes 150-300 uL, 2 columnsAdd to Cart  14.15
803019-50 - Clarion P2 Columns, hydrated gel filtration column for protein purification; for sample volumes 150-300 uL, 50 columnsAdd to Cart  290.09
803020-2 - Clarion P5 Columns, hydrated gel filtration column for protein purification; for sample volumes 100-500 uL, 2 columnsAdd to Cart  15.47
803020-50 - Clarion P5 Columns, hydrated gel filtration column for protein purification; for sample volumes 100-500 uL, 50 columnsAdd to Cart  309.35
803021-2 - Clarion P10 Columns, hydrated gel filtration column for protein purification; for sample volumes 0.5-1.0 mL, 2 columnsAdd to Cart  16.68
803021-50 - Clarion P10 Columns, hydrated gel filtration column for protein purification; for sample volumes 0.5-1.0 mL, 50 columnsAdd to Cart  322.00
803022-2 - Clarion P25 Columns, hydrated gel filtration column for protein purification; for sample volumes up to 2.5 mL, 2 columnsAdd to Cart  20.53
803022-25 - Clarion P25 Columns, hydrated gel filtration column for protein purification; for sample volumes up to 2.5 mL, 25 columnsAdd to Cart  212.75
803023-1 - Clarion P50 Columns, hydrated gel filtration column for protein purification; for sample volumes up to 5.0 mL, 1 columnAdd to Cart  20.64
803023-10 - Clarion P50 Columns, hydrated gel filtration column for protein purification; for sample volumes up to 5.0 mL, 10 columnsAdd to Cart  161.29
803024-1 - Clarion P96 Gel Filtration Column Array, with Sorbadex-25M for protein purification, 1 array of 96Add to Cart  920.00
803025-1 - Clarion P100 Columns, hydrated gel filtration column for protein purification; for sample volumes up to 10.0 mL, 1 columnAdd to Cart  41.06
803025-10 - Clarion P100 Columns, hydrated gel filtration column for protein purification; for sample volumes up to 10.0 mL, 10 columnsAdd to Cart  309.64
Hydrated with pure, deionized water. Pre-swollen, pre-packed and ready-to-use.

Clarion MINI Columns are used for quick and efficient desalting, buffer exchange and/or removal of dyes and small molecules from proteins (S-25 greater than 5 kD, S-50 greater than 25 kD). Purified proteins are eluted into pure, deionized water (Caution! Some proteins may precipitate in pure water with low ionic strength!) The columns are sterile packed, pre-swollen and ready-to-use.
Hydrated with pure deionized water and stabilized with 0.02% Sodium azide. Pre-swollen, pre-packed and ready-to-use.

Clarion MINI Columns are used for quick and efficient desalting, buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD. Purified proteins are eluted into pure, deionized water (Caution! Some proteins may precipitate in pure water with low ionic strength!) The columns are stabilized with 0.02% sodium azide, pre-swollen and ready-to-use.
Hydrated with 1 mM TRIS pH 6. Pre-swollen, pre-packed and ready-to-use.

Clarion TRIS MINI Columns are used for rapid buffer exchange and/or removal of dyes and small molecules from proteins (S-25 greater than 5 kD, S-50 greater than 25kD). Purified proteins are eluted into 1 mM TRIS, pH 6. The columns are sterile packed, pre-swollen and ready-to-use.
Hydrated with Phosphate Buffered Saline pH 7. Pre-swollen, pre-packed and ready-to-use.

Clarion PBS MINI Columns are used for rapid buffer exchange and/or removal of dyes and small molecules from proteins (S-25 greater than 5 kD, S-50 greater than 25 kD). Purified proteins are eluted into Phosphate Buffered Saline (PBS, pH 7). The columns are sterile packed, pre-swollen and ready-to-use.

Clarion P-Series

Hydrated gel filtration columns for protein purification and desalting.
  • Clarion P2 processes sample volumes of 150 to 300 µL
  • Clarion P5 processes sample volumes of 0.5 mL
  • Clarion P10 processes sample volumes of 0.5 mL to 1.0 mL
  • Clarion P25 processes sample volumes of 2.5 mL
  • Clarion P50 processes sample volumes of 5 mL
  • Clarion P100 processes sample volumes of 10 mL

Clarion Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins.

The gel matrix of Clarion is Sorbadex-25, a beaded composite material composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Sorbadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Sorbadex-25 is 5 kD for proteins.
  • Clarion P2 - Proteins larger than 5 kD in a sample volume of up to 200 µL can be purified with an elution volume of 200 to 350 µL.
  • Clarion P5 - Proteins larger than 5 kD in a sample volume of up to 0.5 mL can be purified with an elution volume of up to 1.0 mL.
  • Clarion P10 - Proteins larger than 5 kD in a sample volume of up to 1 mL can be purified with an elution volume of 1.2 to 1.5 mL.
  • Clarion P25 - Proteins larger than 5 kD in a sample volume of up to 2.5 mL can be purified with an elution volume of 2.7 to 3.5 mL.
  • Clarion P50 - Proteins larger than 5 kD in a sample volume of 5 mL can be purified with an elution volume of 6 to 8 mL.
  • Clarion P100 - Proteins larger than 5 kD in a sample volume of 10 mL can be purified with an elution volume of 12 to 15 mL.