Nucleic Acid Desalting Columns



    Clarion™ N-Series Gel Filtration Columns (Gravity Flow)

  • Clarion™ N-Series Gel Filtration Columns are specifically designed for rapid and efficient removal of small molecules from nucleic acids.
  • Ultrapure gel and specially treated sinter frits ensure outstanding resolution and high selectivity.
  • The gel matrix is Sorbadex™-25, a beaded composite material comprised of ultrapure cross-linked dextran. It exhibits high selectivity, high resolution and chemical stability.
  • Molecules purified with Sorbadex™-25 are separated according to size. Smaller molecules pass significantly slower through the columns than larger molecules. Buffer and pH effects on resolution are minimal.
  • The molecular weight cut-off (MWCO) for Sorbadex™-25 is 10 bases for nucleic acids. Oligonucleotides larger than 10 bases are typically purified with 1.5-fold elution volume.

    Clarion™ MINI Spin Columns

  • Clarion™ MINI Spin Columns are used for quick and efficient desalting, buffer exchange, dye terminator removal and removal of labels from labeling reactions. The columns are sterile packed, pre-swollen with purified water and ready-to-use.
  • Clarion™ MINI Spin Columns are designed for purification and desalting of oligonucleotides longer than 20 base pairs and from proteins greater than 20 kD. Convenient centrifugation protocol results in minimal dilution.
  • Clarion™ MINI Spin Columns process samples having a volume between 10 and 100 µL. Optimal purification and recovery is obtained with a sample volume of 50 µL. The volume of the gel bed is 0.8 mL.




Clarion N-series

Catalog #/DescriptionAdd to Cart/List Price
803010-2 - Clarion N2 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes 150-300 uL, 2 columnsAdd to Cart  14.15
803010-50 - Clarion N2 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes 150-300 uL, 50 columnsAdd to Cart  290.09
803011-2 - Clarion N5 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes 100-500 µL, 2 columnsAdd to Cart  15.47
803011-50 - Clarion N5 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes 100-500 uL, 50 columnsAdd to Cart  310.50
803012-2 - Clarion N10 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes 0.5-1.0 mL, 2 columnsAdd to Cart  16.73
803012-50 - Clarion N10 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes 0.5-1.0 mL, 50 columnsAdd to Cart  322.00
803013-2 - Clarion NF10 Gel Filtration Columns, N10 with Sorbadex S-50 Medium, 50ml, 2 columnsAdd to Cart  19.90
803013-50 - Clarion NF10 Gel Filtration Columns, N10 with Sorbadex S-50 Medium, 50ml, 50 columnsAdd to Cart  351.90
803014-2 - Clarion N25 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes up to 2.5 mL, 2 columnsAdd to Cart  20.53
803014-25 - Clarion N25 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes up to 2.5 mL, 25 columnsAdd to Cart  212.75
803015-1 - Clarion N50 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes up to 5.0 mL, 1 columnAdd to Cart  20.64
803015-10 - Clarion N50 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes up to 5.0 mL, 10 columnsAdd to Cart  161.29
803016-1 - Clarion N96 Gel Filtration Column Array with Sorbadex S-25 Medium for nucleic acid purification, 1 array of 96Add to Cart  920.00
803017-1 - Clarion N100 Columns, With Sorbadex S-25 Medium For Desalting, Buffer Exchange, and/or Remove Free Label, 10ml, 1 columnAdd to Cart  41.06
803017-10 - Clarion N100 Columns, With Sorbadex S-25 Medium For Desalting, Buffer Exchange, and/or Remove Free Label, 10ml, 10 columnsAdd to Cart  309.64
803018-1 - Clarion N500 Columns, hydrated gel filtration column for nucleic acid purification; for sample volumes up to 50.0 mL, 1 columnAdd to Cart  235.75


SEQ S-50

Catalog #/DescriptionAdd to Cart/List Price
803007-4 - Clarion MINI Spin Columns, SEQ S-50, hydrated with pure, deionized water for rapid removal of dyes and dideoxy terminators from sequencing reactions and desalting of oligonucleotides greater than 20 base pairs, 4 columnsAdd to Cart  19.38
803007-25 - Clarion MINI Spin Columns, SEQ S-50, hydrated with pure, deionized water for rapid removal of dyes and dideoxy terminators from sequencing reactions and desalting of oligonucleotides greater than 20 base pairs, 25 columnsAdd to Cart  89.13
803007-100 - Clarion MINI Spin Columns, SEQ S-50, hydrated with pure, deionized water for rapid removal of dyes and dideoxy terminators from sequencing reactions and desalting of oligonucleotides greater than 20 base pairs, 100 columnsAdd to Cart  328.33

Hydrated gel filtration columns for nucleic acid purification and desalting.

  • Clarion N2 processes sample volumes of 150 to 300 µL
  • Clarion N5 processes sample volumes of 0.5 mL
  • Clarion N10 processes sample volumes of 0.5 mL to 1.0 mL
  • Clarion N25 processes sample volumes of 2.5 mL
  • Clarion N50 processes sample volumes of 5 mL
  • Clarion N100 processes sample volumes of 10 mL
  • Clarion N500 processes sample volumes of 50 mL

The gel matrix of Clarion is Sorbadex-25, a beaded composite material composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability.

Molecules purified with Sorbadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal.

N2 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from nucleic acids.

The gel matrix of Clarion is Sorbadex-25, a beaded composite material composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Sorbadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Sorbadex-25 is 10 base pairs for oligonucleotides.
  • Clarion N2 - Nucleic acids larger than 10 bp in a sample volume of up to 200 µL can be purified with an elution volume of 200 to 350 µL.
  • Clarion N5 - Nucleic acids larger than 10 bp in a sample volume of up to 0.5 mL can be purified with an elution volume of up to 1.0 mL.
  • Clarion N10 - Nucleic acids larger than 10 bp in a sample volume of up to 1 mL can be purified with an elution volume of 1.2 to 1.5 mL.
  • Clarion N25 - Nucleic acids larger than 10 bp in a sample volume of up to 2.5 mL can be purified with an elution volume of 2.7 to 4.0 mL.
  • Clarion N50 - Nucleic acids larger than 10 bp in a sample volume of 5 mL can be purified with an elution volume of 6 to 8 mL.
  • Clarion N100 - Nucleic acids larger than 10 bp in a sample volume of 10 mL can be purified with an elution volume of 12 to 15 mL.
  • Clarion N100 - Nucleic acids larger than 10 bp in a sample volume of 50 mL can be purified with an elution volume of 65 to 70 mL.

Clarion MINI Spin Columns SEQ S-50
Hydrated with pure, deionized water. Pre-swollen, pre-packed and ready-to-use.

Clarion SEQ S-50 MINI Columns are used for rapid removal of dyes and dideoxy terminators from sequencing reactions and for desalting of oligonucleotides greater than 20 base pairs. Purified nucleic acids are eluted into pure, deionized water. The columns are sterile packed, pre-swollen and ready-to-use.

Clarion MINI Spin Columns Desalt S-25N
Hydrated with pure, deionized water. Pre-swollen, pre-packed and ready-to-use.

Clarion Desalt S-25N MINI Columns are used for rapid removal of salts, dyes and other small molecules from nucleic acids larger than 10 base pairs. Purified nucleic acids are eluted into pure, deionized water. The columns are sterile packed, pre-swollen and ready-to-use.